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A preclinical study on the analgesic and antipyretic properties of an oral dosage formula of herbal drug from ten medicinal plants in the Philippines.


Jennylin B. Carreon,
Melfie E. Bungihan,
Gliceria A. Marzan,
Jherose H. Ampaya,
Aileen P. Baldonado,
Eden Leah B. Bumidang,
Rosanna R. dela Cruz

Related Institution

Center for Natural Sciences - Saint Mary's University

Department of Pharmacy - Saint Mary's University

Publication Information

Publication Type
Research Project
July 1, 2015-March 11, 2016


Ten medicinal plants were listed by the Philippine Department of Health as priority plants and were recognized as herbal plants due to their biological and pharmacological activities. Eight out of these were chosen namely akapulko, ampalaya, bawang, bayabas, lagundi, niyog-niyogan, pansit-pansitan, tsaang-gubat along with sampalok and okra seeds for the formulation of antipyretic and analgesic drugs. Toxicological and pharmacological assays were done on the individual extracts and on the three formulated Liqcap (500 mg/kg) containing different combinations of the individual extracts. Toxicological screening was done on triplicate set-up of Swiss mice (Mus musculus) based on Approximate Lethal Dose (ALD) by single-dose method and pharmacological observations were done on central nervous system (CNS) depression, CNS stimulation, eye observation, ear observation, and general observations and subjective tests. Results showed that after 24 and 48 hours, no CNS depression and stimulation were evident, eyes were normal, ears were normal, general observations and subjective tests were normal. Furthermore, results showed that the individual extracts and the three formulated Liqcap, were not toxic even up to 160 and 1, 500 mg/kg BW, respectively.  Based on the antipyretic assay, results show that Formulation 1 had the best antipyretic effect having a mean decrease of 4.3oC in the rectal temperature of the experimental animals after two hours of treatment. The results further reveal that there is a significant difference in the antipyretic property 30 minutes after the introduction of Formulation 1 to the feverish mice with sig. = 0.002 (α=0.05). Based on the analgesic assay, results present that in Formulation 1, the experimental animals can tolerate pain in the longest time having a mean average of 22.8 seconds. Thus, Formulation 1 containing 100 mg akapulko, 50 mg ampalaya, 140 mg bawang, 135 mg bayabas, 26 mg lagundi, 10 mg niyog-niyogan, 20 mg okra seeds, 5 mg pansit-pansitan, 12 mg sampalok and 2 mg tsaang-gubat can significantly reduce fever and can alleviate pain which is comparable to the over-the-counter synthetic drugs paracetamol and ibuprofen. 


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